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BIOLOGY 220 - Transmission Electron Microscopy

[Go to semester: Spring 2004, Fall 2004]


Theory and principles of the transmission electron microscope (TEM) are taught in this 7-week short course. The TEM enables high-resolution examination of a material's internal features and ultrastructure.

The following TEM micrographs were taken by students.
(below) Murine heart tissue, showing mitochondria (M), lipid droplets (Ld) and muscle tissue (myofibril). (Qi Le '06)

(below, left) Darkly stained mitochondria are distributed throughout this cross-sectional view of cardiac muscle. (John Beers '06)
(below, right) This image of cardiac muscle myofibrils clearly shows alternating isotropic (lighter in color) and anisotropic (longer and more darkly stained) bands. (Doris Luk '05)

(below) Murine kidney tissue; visible are several cell nuclei (N) and lumina (C), red blood cells (e), a podocyte (Po) and the basal lamina (BL). (Doris Luk '05)

(below, left) A podocyte (P) in murine renal tissue, and the 'foot-like' projections (p) of another that give these cells their name. (James Castellano '05)
(below, right) Kidney. This micrograph shows mitochondria overlaid by the deep infoldings of the basal surface of the plasma membrane. (Lilia Zhahalyak IDP)

(below, left) A bile canaliculus (bc) filled with microvilli (mv) can be seen in this micrograph of murine liver. (Lilia Zhahalyak IDP)
(below, right) Endoplasmic reticulum in liver tissue, flecked with ribosomes (R). (Qi Le '06)
(below, left) Murine intestinal tissue. (James Castellano '05)
(below, right) Murine intestine. A closer look at individual microvilli and the internal structure of the tissue. (James Castellano '05)