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BIOLOGY 220 - Transmission Electron Microscopy

[Go to semester: Spring 2004, Fall 2004]

Theory and principles of the transmission electron microscope (TEM) are taught in this 7-week short course. The TEM enables high-resolution examination of a material's internal features and ultrastructure.

The following TEM micrographs were taken by students.

(below, left) An arrangement of cells and cellular components in a sample of murine liver. (Benjamin Rohrer '05)
(below, right)
A closer look at a single round mitochondrion (M) and cell nucleus (N) edged by the endoplasmic reticulum (ER) in murine liver. (Benjamin Rohrer '05)

(below) Rod outer segments (striated ellipses on left of image) and neuronal cytoplasm (gray material on right of image) meet in this sample of murine retina. (Jason Gallant '05)

(below, left) A closer look at several rods in a retinal sample. These cells contain light-sensitive pigments responsive even to dim light sources and help transmit vision-enabling signals to the brain. (Benjamin Rohrer '05)
(below, right)
Fine structure of a single rod cell in a sample of murine retina. Each cell is essentially a stack of thin membranous disks. (Jason Gallant '05)

(below) In this micrograph of murine cardiac muscle, the discontinuous intercalated disc (I) is clearly shown separating sections of fibrillated muscle (M) amongst mitochondria (m). (Jason Gallant '05)