Trace Analysis of Triclosan Using SPE, GC-MS and ELISA

Erica Smith

Research Advisor: Professor David Henderson

Abstract:

Triclosan (5-chloro-2-(2,4-dichlorophenoxy)phenol) is used as a common biocide in commercial products. Water contamination by sewage effluent could be determined by analysis of triclosan levels. Analysis methods for detecting triclosan using SPE, GC-MS and the principles of ELISA are under development and optimization. Using the internal standard, D₃-triclosan, accurate calibrations have been obtained and a limit of detection for the GC-MS method has been determined. Water samples have been analyzed using both GC-MS and ELISA. The final method will be later applied to the study of well contamination in Connecticut.